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Antibodies

MPP8 antibody (mAb)

Clone: N119/44
Aliases: MPHOSPH8, TWA3, HSMPP8
Catalog No: 39819 Format: 100 µg $410 Buy Now
Catalog No: 39820 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:120 kDa
Reactivity:Human

Applications

Western Blot Validated

for MPP8 antibody (mAb) (Clone N119/44)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

for MPP8 antibody (mAb) (Clone N119/44)Immunogen

This MPP8 antibody was raised against a recombinant protein corresponding to amino acids 50-120 of human MPP8 (M-phase phosphoprotein 8).

for MPP8 antibody (mAb) (Clone N119/44)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MPP8 antibody (mAb) (Clone N119/44)References

 
MPP8 antibody (mAb) tested by Western blot.

MPP8 antibody (mAb) tested by Western blot.
Nuclear extract of HEK293 cells (20 µg per lane) probed with MPP8 antibody (mAb) (2 µg/ml dilution).

for MPP8 antibody (mAb) (Clone N119/44)Background

MPP8 (M-Phase Phosphoprotein 8) was originally identified as a phosphoprotein that reacts with MPM2, an antibody that labels mitosis-specific and cell cycle-regulated phosphoproteins. MPP8 contains a single chromodomain that preferentially interacts with nucleosomes enriched for histone H3 lysine 9 methylation but not lysine 4 methylation.

for MPP8 antibody (mAb) (Clone N119/44)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for MPP8 antibody.

for MPP8 antibody (mAb) (Clone N119/44)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MPP8 antibody (mAb) (Clone N119/44)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MPP8 antibody (mAb)
 

Data Thumbnails

MPP8 antibody (mAb) tested by Western blot.

Western blot of MPP8 mAb.
(Click image to enlarge and see details.)