Histone H3 phospho Thr11 antibody (mAb)

for Histone H3 phospho Thr11 antibody mAb (Clone N123/48)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Phosphorylation of several residues of histone H3, such as Ser10 (Histone H3 phospho Ser10), Ser28 (Histone H3 phospho Ser28) and Thr11, is tightly correlated with chromosome condensation during both mitosis and meiosis. Localization of histone H3 phospho Thr11 is different in mammals and in plants. In plant cells, phosphorylation of Thr11 of histone H3 is distributed along the entire length of condensed chromosome. In mammals, this modification is restricted to the centromeric region of the chromosome. Thr11 of histone H3 is phosphorylated by Dlk/ZIP kinases during mitosis.

for Histone H3 phospho Thr11 antibody mAb (Clone N123/48)Immunogen

This Histone H3 phospho Thr11 antibody was raised against a peptide containing phospho-Thr11 of human histone H3.

for Histone H3 phospho Thr11 antibody mAb (Clone N123/48)Application Notes

This Histone H3 phospho Thr11 antibody has been validated for use in Western blotting (0.5 - 2 µg per ml) and immunocytochemsitry.

for Histone H3 phospho Thr11 antibody mAb (Clone N123/48)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for Histone H3 phospho Thr11 antibody mAb (Clone N123/48)Positive Control

Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for Histone H3 phospho Thr11 antibody.

for Histone H3 phospho Thr11 antibody mAb (Clone N123/48)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H3 phospho Thr11 antibody mAb (Clone N123/48)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.