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Antibodies

Histone H3T11ph antibody (mAb)

Clone: N123/48
Catalog No: 39821 Format: 100 µg $410 Buy Now
Catalog No: 39822 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:17 kDa
Reactivity:Human

Applications

Western Blot Validated Dot Blot Validated

for Histone H3 phospho Thr11 antibody (mAb) (Clone N123/48)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

for Histone H3 phospho Thr11 antibody (mAb) (Clone N123/48)Immunogen

This Histone H3 phospho Thr11 antibody was raised against a peptide containing phospho-Thr11 of human histone H3.

for Histone H3 phospho Thr11 antibody (mAb) (Clone N123/48)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3 phospho Thr11 antibody (mAb) (Clone N123/48)References

 
Histone H3T11ph antibody (mAb) tested by Western blot.

Histone H3 phospho Thr11 antibody (mAb) tested by Western blot.
Nuclear extract of HeLa cells (20 µg per lane) probed with Histone H3 phospho Thr11 antibody (mAb) at a dilution of 0.5 µg/ml. Lane 1: No treatment. Lane 2: Cells treated with colcemid.

Histone H3T11ph antibody (mAb) tested by dot blot analysis.

Histone H3 phospho Thr11 antibody (mAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr11 antibody (mAb) for histone H3 phosphorylated at threonine 11. Peptides corresponding to regions around major sites of histone H3 phosphorylation were spotted onto PVDF and probed with Histone H3 phospho Thr11 antibody (mAb) at a dilution of 0.5 µg/ml. The amount of peptide (in picomoles) spotted is indicated next to each row.
Lane 1: peptide phosphorylated at threonine 11. Lane 2: unmodified threonine 11 peptide. Lane 3: peptide phosphorylated at threonine 3. Lane 4: peptide phosphorylated at threonine 6. Lane 5: peptide phosphorylated at threonine 45. Lane 6: peptide phosphorylated at threonine 80. Lane 7: peptide phosphorylated at serine 10. Lane 8: peptide phosphorylated at serine 28.

for Histone H3 phospho Thr11 antibody (mAb) (Clone N123/48)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Phosphorylation of several residues of histone H3, such as Ser10 (Histone H3 phospho Ser10), Ser28 (Histone H3 phospho Ser28) and Thr11, is tightly correlated with chromosome condensation during both mitosis and meiosis. Localization of histone H3 phospho Thr11 is different in mammals and in plants. In plant cells, phosphorylation of Thr11 of histone H3 is distributed along the entire length of condensed chromosome. In mammals, this modification is restricted to the centromeric region of the chromosome. Thr11 of histone H3 is phosphorylated by Dlk/ZIP kinases during mitosis.

for Histone H3 phospho Thr11 antibody (mAb) (Clone N123/48)Positive Control

Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for Histone H3 phospho Thr11 antibody.

for Histone H3 phospho Thr11 antibody (mAb) (Clone N123/48)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 phospho Thr11 antibody (mAb) (Clone N123/48)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3T11ph antibody (mAb)
 

Data Thumbnails

Histone H3T11ph antibody (mAb) tested by Western blot.

Western blot of Histone H3T11ph mAb.
(Click image to enlarge and see details.)

Histone H3T11ph antibody (mAb) tested by dot blot analysis.

Dot blot of Histone H3T11ph mAb.
(Click image to enlarge and see details.)