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Antibodies

HOXA9 antibody (pAb)

Aliases: HOX1, ABD-B, HOX1G, HOX1.7
Catalog No: 39825 Format: 100 µl $410 Buy Now
Catalog No: 39826 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:48 kDa
Reactivity:Human

Applications

Western Blot Validated

for HOXA9 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for HOXA9 antibody (pAb)Immunogen

This HOXA9 antibody was raised against a peptide within the N-terminal region of human HOXA9.

for HOXA9 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for HOXA9 antibody (pAb)References

 
HOXA9 antibody (pAb) tested by Western blot.

HOXA9 antibody (pAb) tested by Western blot.
K-562 nuclear extract (20 µg per lane) probed with HOXA9 antibody at a dilution of 1:1,000.

for HOXA9 antibody (pAb)Background

HOXA9 (Homeobox protein A9, HOX1G) is a sequence-specific DNA binding transcription factor with a central role in both hematopoiesis and leukemia. HOXA9 is required for normal hematopoiesis and contributes to myeloid blood cell differentiation. High levels of HOXA9 expression in hematopoietic cells is a characteristic feature of acute myeloid leukemia (AML), and may contribute to the development of this disease. Overexpression of HOXA9 markedly expands hematopoietic stem cells.

for HOXA9 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for HOXA9 antibody.

for HOXA9 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HOXA9 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HOXA9 antibody (pAb)
 

Data Thumbnails

HOXA9 antibody (pAb) tested by Western blot.

Western blot of HOXA9 pAb.
(Click image to enlarge and see details.)