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Antibodies

HOXA9 antibody (pAb)

Catalog No: 39825 Format: 100 µl $340 Buy Now
Catalog No: 39826 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:WB
Reactivity:Human
Molecular Weight:48 kDa

Applications Table

Western Blot Chromatin IP
1:500 - 1:2,000

for HOXA9 antibody (pAb)Background

HOXA9 (Homeobox protein A9, HOX1G) is a sequence-specific DNA binding transcription factor with a central role in both hematopoiesis and leukemia. HOXA9 is required for normal hematopoiesis and contributes to myeloid blood cell differentiation. High levels of HOXA9 expression in hematopoietic cells is a characteristic feature of acute myeloid leukemia (AML), and may contribute to the development of this disease. Overexpression of HOXA9 markedly expands hematopoietic stem cells.

for HOXA9 antibody (pAb)Immunogen

This HOXA9 antibody was raised against a peptide within the N-terminal region of human HOXA9.

for HOXA9 antibody (pAb)Application Notes

This HOXA9 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution).

Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.

for HOXA9 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for HOXA9 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36015) can be used as a positive control for HOXA9 antibody.

 
HOXA9 antibody (pAb) tested by Western blot.

HOXA9 antibody (pAb) tested by Western blot.
K562 nuclear extract (20 µg per lane) probed with HOXA9 antibody at a dilution of 1:1,000.

for HOXA9 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HOXA9 antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

HOXA9 antibody (pAb)
 

Data Thumbnails

HOXA9 antibody (pAb) tested by Western blot.

Western blot of HOXA9 antibody.
(Click image to enlarge and see details).