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Antibodies

Boris / CTCFL antibody (pAb)

Aliases: CT27, BORIS, CTCF-T, HMGB1L1
Catalog No: 39851 Format: 100 µl $420 Buy Now
Catalog No: 39852 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:96 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for Boris / CTCFL antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 µl per ChIP
ChIP-Seq: 5 µl each
WB: 1:500 - 1:2,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

for Boris / CTCFL antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human BORIS.

for Boris / CTCFL antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Boris / CTCFL antibody (pAb)References

 
Boris / CTCFL antibody (pAb) tested by ChIP-Seq.

Boris / CTCFL antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human testis and 2 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Boris / CTCFL binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

Boris / CTCFL antibody (pAb) tested by Western blot.

BORIS / CTCFL antibody (pAb) tested by Western blot.
Whole cell extract (10 μg per lane) from HEK293T cells transfected with either vector alone (lanes 1 and 3), with a FLAG-Tagged human BORIS expression vector (lanes 2 and 4) or mouse testis nuclear extract (30 μg, lane 5) was probed with either BORIS / CTCFL antibody at 1:2,000 (lanes 1 and 2) or 1:500 (lane 5) or anti-FLAG-Tag (lanes 3 and 4).

for Boris / CTCFL antibody (pAb)Background

BORIS (Brother of Regulator of Imprinted Sites) / CTCFL (CTCF-like) is a zinc-finger DNA binding protein that acts as a chromatin insulator and a paralog of the CTCF protein. Normally, BORIS / CTCFL is expressed only in primary spermatocytes and is thought to play a role in resetting DNA methylation marks during the differentiation of male germ cells. BORIS / CTCFL is also expressed in many cancers and may serve a pro-proliferative function.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Boris / CTCFL antibody (pAb).

for Boris / CTCFL antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Boris / CTCFL antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Boris / CTCFL antibody (pAb)
 

Data Thumbnails

Boris / CTCFL antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Boris / CTCFL pAb.
(Click image to enlarge and see details.)

Boris / CTCFL antibody (pAb) tested by Western blot.

Western blot of Boris / CTCFL pAb.
(Click image to enlarge and see details.)