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Boris / CTCFL antibody (pAb)
| Catalog No: 39851 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39852 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for Boris / CTCFL antibody (pAb)Background
BORIS (Brother of Regulator of Imprinted Sites) / CTCFL (CTCF-like) is a zinc-finger DNA binding protein that acts as a chromatin insulator and a paralog of the CTCF protein. Normally, BORIS / CTCFL is expressed only in primary spermatocytes and is thought to play a role in resetting DNA methylation marks during the differentiation of male germ cells. BORIS / CTCFL is also expressed in many cancers and may serve a pro-proliferative function.
for Boris / CTCFL antibody (pAb)Immunogen
This CTBP antibody was raised against a peptide within the N-terminal region of human BORIS.
for Boris / CTCFL antibody (pAb)Application Notes
This BORIS / CTCFL antibody has been validated for use in Western blotting (1:500 - 1:2,000)
for Boris / CTCFL antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
BORIS / CTCFL antibody (pAb) tested by Western blot.
Whole cell extract (10 μg per lane) from HEK293T cells transfected with either vector alone (lanes 1 and 3), with a FLAG-tagged human BORIS expression vector (lanes 2 and 4) or mouse testis nuclear extract (30 μg, lane 5) was probed with either BORIS / CTCFL antibody at 1:2,000 (lanes 1 and 2) or 1:500 (lane 5) or anti-FLAG tag (lanes 3 and 4).
for Boris / CTCFL antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Boris / CTCFL antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
