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Antibodies

Ago2 antibody (mAb)

Clone: 1B1-E2H5
Catalog No: 39853 Format: 100 µg $340 Buy Now
Catalog No: 39854 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG2a
Purification:Protein G Chromatography
Applications:IF, IP, WB
Reactivity:Human
Molecular Weight:100 kDa

Applications Table

Western Blot Chromatin IP IF IP
0.2 µg/ml — 0.5 - 2 µg/ml 5 µg per IP

for Ago2 antibody (mAb) (Clone 1B1-E2H5)Background

Argonaute 2 (Ago2, aka EIF2C2) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage.

for Ago2 antibody (mAb) (Clone 1B1-E2H5)Immunogen

This Ago2 antibody was raised against a recombinant protein corresponding to amino acids 1-148 of human Ago2.

for Ago2 antibody (mAb) (Clone 1B1-E2H5)Application Notes

This Ago2 antibody has been validated in immunofluorescence (0.5 - 2 µg/ml), immunoprecipitation (5 µg per IP) and Western blotting (0.2 µg/ml).

See references for more information.

for Ago2 antibody (mAb) (Clone 1B1-E2H5)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Ago2 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Ago2 antibody (mAb).

for Ago2 antibody (mAb) (Clone 1B1-E2H5)References

This antibody has been used as described in the following references:
IF: Azuma AM et al (2010) PNAS 105: 7964-7969
IP: Azuma AM et al (2010) PNAS 105: 7964-7969
WB: Azuma AM et al (2010) PNAS 105: 7964-7969

for Ago2 antibody (mAb) (Clone 1B1-E2H5)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Ago2 antibody (mAb) (Clone 1B1-E2H5)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting