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Antibodies

Ago4 antibody (mAb)

Clone: 1B7-G11
Catalog No: 39855 Format: 100 µg $340 Buy Now
Catalog No: 39856 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG
Purification:Protein G Chromatography
Applications:IP, WB
Reactivity:Human
Molecular Weight:100 kDa

Applications Table

Western Blot Chromatin IP Immunoprecipitation
4 µg/ml — 5 µg per IP

for Ago4 antibody (mAb) (Clone 1B7-G11)Background

Argonaute 4 (Ago4, aka EIF2C4) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago4 lacks RNase activity and does not appear to cleave target mRNA molecules. It binds to microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Ago4 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.

for Ago4 antibody (mAb) (Clone 1B7-G11)Immunogen

This Ago4 antibody was raised against a recombinant protein corresponding to amino acids 1-164 of human Ago4.

for Ago4 antibody (mAb) (Clone 1B7-G11)Application Notes

This Ago4 antibody has been validated in immunoprecipitation (5 µg per IP) and Western blotting (4 µg/ml).

See references for more information.

for Ago4 antibody (mAb) (Clone 1B7-G11)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Ago4 antibody (mAb).

for Ago4 antibody (mAb) (Clone 1B7-G11)References

This antibody has been used as described in the following references:
IP: Azuma AM et al (2010) PNAS 105: 7964-7969
WB: Azuma AM et al (2010) PNAS 105: 7964-7969

for Ago4 antibody (mAb) (Clone 1B7-G11)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Ago4 antibody (mAb) (Clone 1B7-G11)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting