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Antibodies

MBD1 antibody (pAb)

Aliases: DPEP1, MDP, RDP
Catalog No: 39857 Format: 100 µl $410 Buy Now
Catalog No: 39858 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:80 kDa
Reactivity:Human

Applications

Western Blot Validated

for MBD1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

for MBD1 antibody (pAb)Immunogen

This MBD1 antibody was raised against a peptide within the C-terminal region of human MBD1.

for MBD1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MBD1 antibody (pAb)References

 
MBD1 antibody (pAb) tested by Western blot.

MBD1 antibody tested by Western blot.
Western blot using MBD1 antibody (pAb) at 1:500 dilution. Lane 1: Nuclear extract (25 µg) of HeLa cells. Lane 2: Nuclear extract (30 µg) of Jurkat cells.

for MBD1 antibody (pAb)Background

MBD1 (methyl-CpG binding domain protein 1) is thought to function as a mediator of the biological consequences of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD1 is recruited to both methylated and non-methylated CpGs via separate domains: the CXXC domain targets the protein to non-methylated CpGs whereas the MBD domain targets the protein to methylated CpG. During S phase of the cell cycle, MBD1 can interact with SETDB1 (H3K9 methyltransferase).

for MBD1 antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for MBD1 antibody.

for MBD1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MBD1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MBD1 antibody (pAb)
 

Data Thumbnails

MBD1 antibody (pAb) tested by Western blot.

Western blot of MBD1 pAb.
(Click image to enlarge and see details.)