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Antibodies

MMSET / WHSC1 antibody (mAb)

Clone: 29D1
Aliases: WHS, NSD2, TRX5, REIIBP
Catalog No: 39879 Format: 100 µg $420 Buy Now
Catalog No: 39880 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:88 & 157 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for MMSET / WHSC1 antibody (mAb) (Clone 29D1)Application Notes


Validated Applications:
ChIP: 2 - 5 µg per ChIP
ChIP-Seq: 10 µg each
ICC/IF: 0.5 µg/ml dilution

Published Applications:
WB
See references for more information. Individual optimization may be required.

for MMSET / WHSC1 antibody (mAb) (Clone 29D1)Immunogen

This MMSET / WHSC1 antibody was raised against a recombinant protein corresponding to amino acids 1-647 of human MMSET-I.

for MMSET / WHSC1 antibody (mAb) (Clone 29D1)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MMSET / WHSC1 antibody (mAb) (Clone 29D1)References

 
MMSET / WHSC1 antibody (mAb) tested by ChIP-Seq.

MMSET / WHSC1 antibody (mAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human multiple myeloma cell line (4.5 million cells) and 10 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 23 million sequence tags were mapped to identify MMSET binding patterns. The panel on the left shows binding across 12 million bp on chromosome 12. The panel on the right is zoomed in to show individual peaks.

MMSET / WHSC1 antibody (mAb) tested by immunofluorescence.

MMSET / WHSC1 antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with MMSET / WHSC1 antibody at a 0.5 µg/ml dilution.

for MMSET / WHSC1 antibody (mAb) (Clone 29D1)Background

MMSET (Multiple Myeloma SET domain-containing protein) / WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1; also known as NSD2, Nuclear Receptor binding SET domain protein 2; TRX5, Trithorax 5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The MMSET / WHSC1 gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15% of patients with Multiple Myeloma (MM), a chromosomal translocation leads to MMSET / WHSC1 gene being aberrantly placed under the control of the IgH intronic µ enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in MMSET / WHSC1 expression in a myeloma cell line suppressed its growth, suggesting a role for MMSET in maintaining the transformed state.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with MMSET / WHSC1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use MMSET / WHSC1 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with MMSET / WHSC1 antibody (mAb).

for MMSET / WHSC1 antibody (mAb) (Clone 29D1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MMSET / WHSC1 antibody (mAb) (Clone 29D1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MMSET / WHSC1 antibody (mAb)
 

Data Thumbnails

MMSET / WHSC1 antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of MMSET / WHSC1 mAb.
(Click image to enlarge and see details.)

MMSET / WHSC1 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of MMSET / WHSC1 mAb.
(Click image to enlarge and see details.)