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Antibodies

Histone H3K9me1 antibody (pAb)

Catalog No: 39887 Format: 100 µg $420 Buy Now
Catalog No: 39888 Format: 10 µg $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Dot Blot Validated

for Histone H3 monomethyl Lys9 antibody (pAb)Application Notes


Validated Applications:
ChIP: 4 µg per ChIP
ChIP-Seq & ChIP-chip: 4 µg each
WB: 1 - 2 µg/ml dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for Histone H3 monomethyl Lys9 antibody (pAb)Immunogen

This Histone H3 monomethyl Lys9 antibody was raised against a peptide including monomethyl lysine 9 of histone H3.

for Histone H3 monomethyl Lys9 antibody (pAb)Buffer

Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39249) of this antibody is also available.

for Histone H3 monomethyl Lys9 antibody (pAb)References

 
Histone H3K9me1 antibody (pAb) tested by ChIP-chip.

Histone H3K9me1 antibody (pAb) tested by ChIP-chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human breast cancer cell line (4.5 million cells) and 4 µl of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The literature suggests that this histone modification is enriched in actively transcribed genes. The 2 images above show enrichment of H3K9me1 across gene bodies.

Histone H3K9me1 antibody (pAb) tested by Western blot.

Histone H3 monomethyl Lys9 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (20 µg per lane) probed with Histone H3 monomethyl Lys9 antibody at a dilution of 2 µg/ml.

Histone H3K9me1 antibody (pAb) tested by dot blot analysis.

Histone H3 monomethyl Lys9 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys9 antibody for monomethyl Lys9 of histone H3. Recombinant methylated proteins corresponding to the immunogen and related proteins were spotted onto PVDF and probed with Histone H3 monomethyl Lys9 antibody at 2 µg/ml. The amount of protein (picomoles) spotted is indicated next to each row.

Lane 1: recombinant monomethyl lysine 9 protein.
Lane 2: recombinant dimethyl lysine 9 protein.
Lane 3: recombinant trimethyl lysine 9 protein.

for Histone H3 monomethyl Lys9 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

for Histone H3 monomethyl Lys9 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 monomethyl Lys9 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K9me1 antibody (pAb).

for Histone H3 monomethyl Lys9 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 monomethyl Lys9 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K9me1 antibody (pAb)
 

Data Thumbnails

Histone H3K9me1 antibody (pAb) tested by ChIP-chip.

ChIP-chip of Histone H3K9me1 pAb.
(Click image to enlarge and see details.)

Histone H3K9me1 antibody (pAb) tested by Western blot.

Western blot of Histone H3K9me1 pAb.
(Click image to enlarge and see details.)

Histone H3K9me1 antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3K9me1 pAb.
(Click image to enlarge and see details.)