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Antibodies

DNMT3B antibody (pAb)

Aliases: MmuIIIB
Catalog No: 39899 Format: 100 µl $410 Buy Now
Catalog No: 39900 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:110 kDa
Reactivity:Mouse

Applications

Western Blot Validated

for DNMT3B antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications:
WB:

for DNMT3B antibody (pAb)Immunogen

This DNMT3B antibody was raised against a recombinant protein corresponding to amino acids 1-218 of mouse DNMT3B.

for DNMT3B antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for DNMT3B antibody (pAb)References

 
DNMT3B antibody (pAb) tested by Western blot.

DNMT3B antibody (pAb) tested by Western blot.
Nuclear extract (30 µg) of mouse ES cells probed with DNMT3B antibody at a dilution of 1:500.

for DNMT3B antibody (pAb)Background

DNMT3BDNA methyltransferase (or DNMT 3B) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3.

The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3B has been shown to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.

for DNMT3B antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DNMT3B antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DNMT3B antibody (pAb)
 

Data Thumbnails

DNMT3B antibody (pAb) tested by Western blot.

Western blot of DNMT3B pAb.
(Click image to enlarge and see details.)