SIRT2 antibody (pAb)

for SIRT2 antibody (pAb)Background

SIRT2 is a member of the SIR2 / Sirtuin family of NAD-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, P53, HOXA10, FOXO3A, P300, FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARγ and subsequent repression of PPARγ mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates H4AcK16 similar to SIRT1, SIRT2 deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus and in contact with chromatin and required for exit from mitosis.

for SIRT2 antibody (pAb)Immunogen

This SIRT2 antibody was raised against a synthetic peptide derived from the C-terminal region of human SIRT2.

for SIRT2 antibody (pAb)Application Notes

This SIRT2 antibody has been validated for use in Western blotting (1:500 - 1:2,000).

This antibody recognizes multiple isoforms of SIRT2, including two species of 40 and 45 kDa.

Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.

for SIRT2 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for SIRT2 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for SIRT2 antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.