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Antibodies

SIRT2 antibody (pAb)

Aliases: SIR2, SIR2L, SIR2L2
Catalog No: 39903 Format: 100 µl $410 Buy Now
Catalog No: 39904 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:40 & 45 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for SIRT2 antibody (pAb)Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for SIRT2 antibody (pAb)Immunogen

This SIRT2 antibody was raised against a synthetic peptide derived from the C-terminal region of human SIRT2.

for SIRT2 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SIRT2 antibody (pAb)References

 
SIRT2 antibody (pAb) tested by immunoprecipitation.

SIRT2 antibody (pAb) tested by Immunoprecipitation.
10 µl of SIRT2 antibody was used to immunoprecipitate SIRT2 from 250 µg of K-562 cytoplasmic cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SIRT2 antibody at a dilution of 1:500.

SIRT2 antibody (pAb) tested by Western blot.

SIRT2 antibody (pAb) tested by Western blot.
Cytoplasmic extract of K-562 cells (25 µg per lane) probed with SIRT2 antibody (1:500).

for SIRT2 antibody (pAb)Background

SIRT2 is a member of the SIR2 / Sirtuin family of NAD-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, P53, HOXA10, FOXO3A, P300, FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARγ and subsequent repression of PPARγ mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates H4AcK16 similar to SIRT1, SIRT2 deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus and in contact with chromatin and required for exit from mitosis.

for SIRT2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SIRT2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SIRT2 antibody (pAb)
 

Data Thumbnails

SIRT2 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of SIRT2 pAb.
(Click image to enlarge and see details.)

SIRT2 antibody (pAb) tested by Western blot.

Western blot of SIRT2 pAb.
(Click image to enlarge and see details.)