DNMT3L antibody (pAb)

for DNMT3L antibody (pAb)Background

DNMT3L (DNA methyltransferase 3-like) is a nuclear protein with similarity to the DNMT family of proteins, but does not function as a DNA methyltransferase. It serves as an essential regulator of DNMT3A and DNMT3B, recruiting them to sites for deposition of de novo DNA methylation. DNMT3L is expressed in the presumptive gametes in mammalian embryos and is required for the establishment of genomic imprinting. DNMT3L has a PHD-like domain that allows binding to chromatin through interactions with the amino terminal tail of histone H3 that is devoid of lysine 4 methylation.

for DNMT3L antibody (pAb)Immunogen

This DNMT1 antibody was raised against a recombinant protein corresponding to full length human DNMT3L.

for DNMT3L antibody (pAb)Application Notes

This DNMT3L antibody has been validated for use in Western blotting (1:500 - 1:2,000)

Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.

for DNMT3L antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for DNMT3L antibody (pAb)Positive Control

Active Motif's Human Testes nuclear extract (Catalog No. 36047) can be used as a positive control for DNMT3L antibody antibody.

for DNMT3L antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for DNMT3L antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.