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BRD4 antibody (pAb)
| Catalog No: 39909 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39910 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | 5 - 10 µl per ChIP |
for BRD4 antibody (pAb)Background
BRD4 (Bromodomain-containing protein 4, aka HUNK1) is a chromatin-associated protein with two bromo domains that binds acetylated histone H3 and H4. BRD4 is homologous to Xenopus MCAP, and like MCAP, BRD4 associates with chromosomes during mitosis, predominantly in the non-centromeric regions. A chromosomal aberration involving BRD4 (producing a BRD4-NUT fusion protein) is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. BRD4 is involved in the expression of extracellular matrix gene expression, and its misregulation may be involved in breast cancer progression.
for BRD4 antibody (pAb)Immunogen
This BRD4 antibody was raised against a peptide within the C-terminal region of human BRD4.
for BRD4 antibody (pAb)Application Notes
This BRD4 antibody has been validated for use in Western blotting (1:500 - 1:1,000) and chromatin IP (5 - 10 per µl ChIP).
for BRD4 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for BRD4 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for BRD4 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with BRD4 antibody (pAb).
BRD4 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (Lane 1, 40 µg) and nuclear extract of P19 cells (Lane 2, 20 µg) probed with BRD4 antibody (pAb) at a dilution of 1:500.
BRD4 antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using Active Motif's Epigenetic Services protocol with 10 µg of chromatin from TNF-α treated HT-29 colorectal adenocarcinoma cells and 5 µl BRD4 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
for BRD4 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for BRD4 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
