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Histone H3 dimethyl Lys4 antibody (pAb)
| Catalog No: 39913 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39914 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 0.5 - 2 µg/ml | 10 µg per ChIP | 1 - 2 µg/ml |
for Histone H3 dimethyl Lys4 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.
Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.
for Histone H3 dimethyl Lys4 antibody (pAb)Immunogen
This Histone H3 dimethyl Lys4 antibody was raised against a peptide including dimethyl-lysine 4 of histone H3.
for Histone H3 dimethyl Lys4 antibody (pAb)Application Notes
This Histone H3 dimethyl Lys4 antibody has been validated for use in chromatin immunoprecipitation (ChIP, 10 µg per ChIP) and Western blotting (0.5 - 2 µg/ml).
for Histone H3 dimethyl Lys4 antibody (pAb)Buffer
PBS pH 7.5, 30% glycerol, 0.035% sodium azide.
for Histone H3 dimethyl Lys4 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 dimethyl Lys4 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 dimethyl Lys4 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 dimethyl Lys4 antibody (pAb).
Histone H3 dimethyl Lys4 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (20 µg) probed with Histone H3 dimethyl Lys4 antibody (1 µg/ml dilution).
Histone H3 dimethyl Lys4 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys4 antibody for dimethyl-Lys4 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with Histone H3 dimethyl Lys4 antibody at a dilution of 1 µg/ml. The amount of peptide (in picomoles) spotted is indicated next to each row.
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Histone H3 dimethyl Lys4 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µg of Histone H3 dimethyl Lys4 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Histone H3 dimethyl Lys4 antibody tested by immunofluorescence.
HeLa cells stained at 2 µg/ml with Histone H3 dimethyl Lys4 antibody. Top panel: Histone H3 dimethyl Lys4 antibody. Middle panel: DAPI. Bottom panel: merge.
for Histone H3 dimethyl Lys4 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 dimethyl Lys4 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
