SREBP-2 antibody (mAb)

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Background

SREBP-1 and SREBP-2 (Sterol regulatory element-binding protein 1 and 2) are members of the basic-helix-loop-helix-leucine zipper (bHLH-ZIP) family of transcription factors and are involved in the regulation of cholesterol homeostasis. SREBP-1 and SREBP-2 are normally membrane bound at the ER and nuclear envelope, but in sterol-depleted cells, the membrane-bound precursors are cleaved to generate a soluble isoforms that translocate to the nucleus. These active forms, which include the bHLH-ZIP domain, activate transcription of genes involved in the uptake and synthesis of cholesterol.

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Immunogen

This SREBP-2 antibody was raised against a recombinant protein corresponding to amino acids 833-1141 of human SREBP-2.

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Application Notes

This SREBP-2 antibody has been validated for use in chromatin immunoprecipitation (ChIP, 1 - 2 µg per ChIP), immunoprecipitation (1 - 2 µg per IP) and Western blotting (0.5 - 2 µg/ml). This antibody recognizes the 125 kDa precursor and the processed 68 kDa nuclear form.

For more information, see References below.

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for SREBP-2 antibody (mAb) (Clone IgG-1C6)References

This antibody has been used in the following publications:
ChIP: Garver WS et al (2008) J Lipid Res 49: 1090-1102.
WB: Swinnen JV et al (1997) PNAS 94: 12975-12980.

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.