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Antibodies

Lamin A/C R482W antibody (mAb)

Clone: 5H8-B4
Catalog No: 39963 Format: 100 µg $340 Buy Now
Catalog No: 39964 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1
Purification:Protein G Chromatography
Applications:IF, IP, WB
Reactivity:Human
Molecular Weight:65 kDa

Applications Table

Western Blot Chromatin IP IF IP
0.5 - 2 µg/ml — 0.5 - 2 µg/ml 2 - 10 µg per IP

for Lamin A/C R482W antibody (mAb) (Clone 5H8-B4)Background

Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization (laminopathy) results in disruption of DNA replication, transcription and RNA processing. The R482W mutation is involved in Dunnigan-type familial partial lipodystrophy (FPLD), a rare form of insulin resistance.

for Lamin A/C R482W antibody (mAb) (Clone 5H8-B4)Immunogen

This Lamin A/C R482W antibody was raised against a recombinant protein corresponding to amino acids 430-545 of human Lamin A/C containing the R482W substitution.

for Lamin A/C R482W antibody (mAb) (Clone 5H8-B4)Application Notes

This Lamin A/C R482W antibody has been validated for use in immunofluorescence (0.5 - 2 µg/ml), immunoprecipitation (2 - 10 µg per IP) and Western blotting (0.5 - 2 µg/ml). See references for more details.

for Lamin A/C R482W antibody (mAb) (Clone 5H8-B4)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Lamin A/C R482W antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Lamin A/C R482W antibody (mAb).

for Lamin A/C R482W antibody (mAb) (Clone 5H8-B4)References

This antibody has been used in the following references:
IF, IP & WB: Roblek M et al (2010) PLoS One 5: e10604.

for Lamin A/C R482W antibody (mAb) (Clone 5H8-B4)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Lamin A/C R482W antibody (mAb) (Clone 5H8-B4)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting