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Antibodies

HP1β antibody (mAb)

Clone: 1MOD-1A9
Aliases: CBX, M31, MOD1, p25beta, HP1Hsbeta, HP1Hs-beta
Catalog No: 39979 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:25 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for HP1b antibody (mAb) (Clone 1MOD-1A9)Application Notes


Published Applications:
IP
ICC/IF
WB
See references for more information. Individual optimization may be required.

for HP1b antibody (mAb) (Clone 1MOD-1A9)Immunogen

This HP1β antibody was raised against recombinant mouse HP1β.

for HP1b antibody (mAb) (Clone 1MOD-1A9)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for HP1b antibody (mAb) (Clone 1MOD-1A9)References

for HP1b antibody (mAb) (Clone 1MOD-1A9)Background

HP1 beta (Heterochromatin Protein 1 beta, Chromobox protein 1, CBX1) is one of the mammalian homologues of Drosophila HP1, first identified through its role in the maintenance of regions of the genome in a heterochromatic state. HP1 beta contains a chromo domain and a chromo shadow domain. Through the chromo domain, HP1 proteins are recruited to regions of chromatin that exhibit methylation of lysine 9 of histone H3. Interaction between HP1 beta and the lamin B receptor can facilitate the sequestration of heterochromatin to the nuclear envelope.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use HP1β antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HP1β antibody (mAb).

for HP1b antibody (mAb) (Clone 1MOD-1A9)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HP1b antibody (mAb) (Clone 1MOD-1A9)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HP1β antibody (mAb)