DAX-1 / NR0B1 antibody (mAb)

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Background

DAX-1 (DSS-AHC critical region on the X chromosome protein 1, NR0B1, Nuclear receptor subfamily 0 group B member 1) is an orphan nuclear receptor and a component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. DAX-1 / NR0B1 inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. It may also have a role in embryonic development and in the maintenance of embryonic stem cell pluripotency as it up-regulates Oct-4 expression ES cells. Defects in DAX-1 are a cause of dosage-sensitive sex reversal (DSSR) and X-linked adrenal hypoplasia congenital (XL-AHC). DSSR results from a duplication of the region of the X-chromosome containing DAX-1, resulting in males with two copies of DAX-1, which inhibits normal testis formation, and thus XY individuals develop as females. XL-AHC is a developmental disorder of the adrenal gland, characterized by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Immunogen

This DAX-1 / NR0B1 antibody was raised against a peptide corresponding to amino acids 135-166 of human DAX-1 / NR0B1.

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Application Notes

This DAX-1 / NR0B1 antibody has been validated for use in ELISA, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation and Western blotting. See references for more information.

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)References

This antibody has been used in the following references:
IF: Battista MC et al (2005) J Clin Endo Metabol 90: 5426-5431.
IHC: Battista MC et al (2005) J Clin Endo Metabol 90: 5426-5431.
WB: Battista MC et al (2005) J Clin Endo Metabol 90: 5426-5431.

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.