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Antibodies

PLZF antibody (mAb)

Clone: 2A9
Aliases: Promyelocytic Leukaemia Zinc Finger, Zinc Finger And BTB Domain Containing 16, zbtb16
Catalog No: 39987 Format: 100 µg $410 Buy Now
Catalog No: 39988 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:90 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for PLZF antibody (mAb) (Clone 2A9)Application Notes


Published Applications:
ChIP
IP
ICC/IF
WB
See references for more information. Individual optimization may be required.

for PLZF antibody (mAb) (Clone 2A9)Immunogen

This PLZF antibody was raised against a recombinant protein corresponding to full-length mouse PLZF with a GST-Tag.

for PLZF antibody (mAb) (Clone 2A9)Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PLZF antibody (mAb) (Clone 2A9)References

for PLZF antibody (mAb) (Clone 2A9)Background

PLZF (Promyelocytic Leukemia Zinc-Finger protein) is a member of the POK (POZ and Krüppel) family of transcriptional repressors. PLZF is expressed in early, but not differentiated, hematopoietic cells, and is required for spermatogonial stem cell maintenance. In addition, PLZF regulates genes involved in cellular proliferation and differentiation (cyclin A2, Myc and Hox genes) and targeted deletion of PLZF in mice results in limb and axial skeleton and limb deformations.

for PLZF antibody (mAb) (Clone 2A9)qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Hoxc10 (Cat. No. 71019) and Mouse Negative Control Primer Set 3 (Cat. No. 71013) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with PLZF antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use PLZF antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with PLZF antibody (mAb).

for PLZF antibody (mAb) (Clone 2A9)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PLZF antibody (mAb) (Clone 2A9)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PLZF antibody (mAb)