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Antibodies

CHD1 antibody (mAb)

Clone: 2F11
Catalog No: 39989 Format: 100 µg $410 Buy Now
Catalog No: 39990 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:210 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for CHD1 antibody (mAb) (Clone 2F11)Application Notes


Published Applications:
ICC/IF
WB
See references for more information. Individual optimization may be required.

for CHD1 antibody (mAb) (Clone 2F11)Immunogen

This CHD1 antibody was raised against a peptide corresponding to the C-terminus of mouse CHD1.

for CHD1 antibody (mAb) (Clone 2F11)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CHD1 antibody (mAb) (Clone 2F11)References

for CHD1 antibody (mAb) (Clone 2F11)Background

CHD1 (Chromo-ATPase/Helicase-DNA-binding protein 1 ) is a member of the CHD family of ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation. They are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD1 has been shown to interact with histone H3 trimethylated at lysine 4 (H3K4Me3) and also is required for the maintenance of stem cell pluripotency.

for CHD1 antibody (mAb) (Clone 2F11)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CHD1 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CHD1 antibody (mAb).

for CHD1 antibody (mAb) (Clone 2F11)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CHD1 antibody (mAb) (Clone 2F11)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CHD1 antibody (mAb)