Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

BMI-1 antibody (mAb)

Clone: AF27
Catalog No: 39993 Format: 100 µg $340 Buy Now
Catalog No: 39994 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG2a
Purification:Protein G Chromatography
Applications:ChIP, IP
Reactivity:Human, Mouse
Molecular Weight:40 kDa

Applications Table

Western Blot Chromatin IP Immunoprecipitation
Not recommended.
WB-validated version available: 61089.		 2 - 10 µg per ChIP 2 - 10 µg per IP

for BMI-1 antibody (mAb) (Clone AF27)Background

BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.

for BMI-1 antibody (mAb) (Clone AF27)Immunogen

This BMI-1 antibody was raised against a recombinant protein corresponding to full length mouse BMI-1.

for BMI-1 antibody (mAb) (Clone AF27)Application Notes

This BMI-1 antibody has been validated for use in chromatin IP (ChIP, 2 - 10 µg per ChIP) and immunoprecipitation (2 - 10 µg per IP). See references for details.

for BMI-1 antibody (mAb) (Clone AF27)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Chromatin IP Assays

Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with BMI-1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use BMI-1 antibody (mAb).

for BMI-1 antibody (mAb) (Clone AF27)References

This antibody has been used in the following references:
ChIP: Bracken AP et al (2007) Genes Dev 21: 535-540; Pasini D et al (2007) Molec Cell Biol 27: 3769-3779.

for BMI-1 antibody (mAb) (Clone AF27)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for BMI-1 antibody (mAb) (Clone AF27)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting