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Antibodies

BMI-1 antibody (mAb)

Clone: AF27
Aliases: PCGF4, RNF51, FLVI2/BMI1
Catalog No: 39993 Format: 100 µg $425 Buy Now
Catalog No: 39994 Format: 10 µg $99 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:40 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for BMI-1 antibody (mAb) (Clone AF27)Application Notes


Validated Applications:
ChIP-Seq: 4 µg per ChIP
WB: 0.5 - 2 µg/mL

For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.

Published Applications:
ChIP
WB
IHC
IF


See references for more information.

Also available as an AbFlex® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91195.

for BMI-1 antibody (mAb) (Clone AF27)Immunogen

This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.

for BMI-1 antibody (mAb) (Clone AF27)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for BMI-1 antibody (mAb) (Clone AF27)References

 
BMI-1 antibody (mAb) tested by ChIP-Seq.

BMI-1 antibody (mAb) tested by ChIP-Seq.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from mouse embryonic fibroblast (MEF) cells and 4 µg BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify BMI-1 binding sites.

BMI-1 antibody (mAb) tested by Western blot.

BMI-1 antibody (mAb) tested by Western blot.
20 µg of K562 cell nuclear extract was run on SDS-PAGE and probed with BMI-1 antibody at 0.5 µg/ml. For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.

for BMI-1 antibody (mAb) (Clone AF27)Background

BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.

Learn more about the PRC1 Complex and Stem Cell Identity

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with BMI-1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use BMI-1 antibody (mAb).

for BMI-1 antibody (mAb) (Clone AF27)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for BMI-1 antibody (mAb) (Clone AF27)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

BMI-1 antibody (mAb)
 

Data Thumbnails

BMI-1 antibody (mAb) tested by ChIP-Seq.

BMI-1 antibody (mAb) tested by ChIP-Seq.
(Click to enlarge and view details)

BMI-1 antibody (mAb) tested by Western blot.

Western blot of BMI-1 mAb.
(Click image to enlarge and see details.)