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Cell, Acid & Tissue Extracts

THP-1 nuclear extract (TPA stimulated)

Catalog No: 40700 Format: 200 µg $165 Buy Now

for THP-1 nuclear extract (TPA stimulated)Contents

2 x 100 µg of THP-1 nuclear extract (PMA stimulated) at 2.5 µg/µl.

for THP-1 nuclear extract (TPA stimulated)Background

THP-1 (TPA stimulated) nuclear extract was prepared from a cell culture of the human acute monocytic leukemia THP-1 cell line. The THP-1 cell line was originally derived from the peripheral blood of a 1 year old body with acute monocytic leukemia. THP-1 cells share similar properties to human monocyte-derived macrophages. These cells differentiate into mature macrophages (phagocytes) when grown in the presence of DMSO or phorbol 12-myristate 13-acetate (TPA, also known as PMA). When stimulated with DMSO or vitamin D3, THP-1 cells adhere to the walls of cell culture flasks, and are therefore frequently utilized as in vitro model systems to study foam cell formation in relation to atherosclerosis. The THP-1 cell line is characterized by the expression of Fc and C3b receptors, the production of IL-1, and the lack expression of cytoplasmic and surface immunoglobulins. In addition, these cells are phagocytic, produce lysosymes and stain positive for alpha-napthyl butyrate esterase. Because of these properties, they are often used in research to study monocyte differentiation.

for THP-1 nuclear extract (TPA stimulated)Application Notes

THP-1 nuclear extract (TPA stimulated) is recommended for use in studies related to 1) blood monocytes, 2) monocyte differentiation and 3) atherosclerosis.

for THP-1 nuclear extract (TPA stimulated)Extract Origin

Human monocytes

for THP-1 nuclear extract (TPA stimulated)Extract Composition

THP-1 nuclear extract was collected in Lysis Buffer after a 24-hour incubation with PMA (10 ng/ml). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay.

for THP-1 nuclear extract (TPA stimulated)Quality Control

Extracts have been quality control tested by Western blot.

for THP-1 nuclear extract (TPA stimulated)Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for THP-1 nuclear extract (TPA stimulated)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

THP-1 nuclear extract (TPA stimulated)