Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

A20 antibody (mAb)

Clone: 59A426
Aliases: TNFAIP3, TNFA1P2, OTUD7C
Catalog No: 40901 Format: 100 µg $410 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:70 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for A20 antibody (mAb) (Clone 59A426)Application Notes


Validated Applications:
WB: 3 µg/ml dilution


Published Applications:
WB



For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for A20 antibody (mAb) (Clone 59A426)Immunogen

This A20 antibody was raised against a full-length protein corresponding to human A20.

for A20 antibody (mAb) (Clone 59A426)Buffer

PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

for A20 antibody (mAb) (Clone 59A426)References

 
A20 antibody (mAb) tested by Western blot.

A20 mAb tested by Western blot.
Detection of A20 by Western blot. A20 can be detected in Daudi whole-cell extract at 3 µg/ml.

for A20 antibody (mAb) (Clone 59A426)Background

A20 is a zinc finger protein induced by Tumor Necrosis Factor (TNF) and involved in limiting inflammation through inhibition of NFκB (NFκB p50 and NFκB p65) activation. A20 also inhibits TNF-mediated apoptosis. A20 is also known as TNFA1P2, TNFAIP3 and OTUD7C.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use A20 antibody (mAb).

for A20 antibody (mAb) (Clone 59A426)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for A20 antibody (mAb) (Clone 59A426)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

A20 antibody (mAb)
 

Data Thumbnails

A20 antibody (mAb) tested by Western blot.

Western blot of A20 mAb.
(Click image to enlarge and see details.)