IκBα phospho Ser32,36 antibody (mAb)

for IkBa phospho Ser32,36 antibody (mAb)Background

IκBα phospho Ser32,36 – NFκB (NFκB p50 and NFκB p65) signaling is controlled to a large extent by the sequestration of the NFκB complex in the cytoplasm by its association with one of the IκB family of proteins. IκBα is phosphorylated at Ser32 and Ser36 by the IκB Kinase (IKK) complex, resulting in the degradation of IκB and the nuclear translocation of NFκB.

for IkBa phospho Ser32,36 antibody (mAb)Immunogen

This antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IκBα.

for IkBa phospho Ser32,36 antibody (mAb)Application Notes

This IκBα phospho Ser32,36 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for IkBa phospho Ser32,36 antibody (mAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IkBa phospho Ser32,36 antibody (mAb)Positive Control

Extracts from Jurkat cells treated for 30 minutes with 100 µg/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal), followed by incubation with or without 1 nM TNF-α cell extract can be used as a positive control for IκBα phospho Ser32, 36 antibody.

for IkBa phospho Ser32,36 antibody (mAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for IkBa phospho Ser32,36 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.