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Antibodies

IKKβ antibody (mAb)

Clone: 10AG2
Aliases: IKBKB, IKK2, IMD15, NFKBIKB, IKK-beta
Catalog No: 40906 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:87 kDa
Reactivity:Human

Applications

Western Blot Validated

for IKKb antibody (mAb) (Clone 10AG2)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

Published Applications: see references

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for IKKb antibody (mAb) (Clone 10AG2)Immunogen

This IKKβ antibody was raised against a 6xHis-Tag full-length protein corresponding to human IKKβ.

for IKKb antibody (mAb) (Clone 10AG2)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IKKb antibody (mAb) (Clone 10AG2)References

 
IKKβ antibody (mAb) tested by Western blot.

IKKβ mAb (Clone 10AG2) tested by Western blot.
IKKβ detection by Western blot analysis. IKKβ can be detected in 30 µg of Daudi whole-cell extract with IKKβ mAb (Clone 10AG2) at a 1 µg/ml dilution.

for IKKb antibody (mAb) (Clone 10AG2)Background

IKKβ (IκB Kinase beta subunit) – The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB (NFκB p50 and NFκB p65). The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ).

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IκBα at Ser32 and Ser36. This results ultimately in the degradation of IκB and the activation and nuclear translocation of NFκB.

While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK beta appears to be the principal kinase, as IKKα is not required for activation of the IKK complex and the subsequent degradation of IκBα. Once activated, IKKβ autophosphorylates, which decreases IKK activity, thereby preventing a prolonged activation of inflammation.

for IKKb antibody (mAb) (Clone 10AG2)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for IKKb antibody (mAb) (Clone 10AG2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

IKKβ antibody (mAb)
 

Data Thumbnails

IKKβ antibody (mAb) tested by Western blot.

Western blot of IKKβ mAb.
(Click image to enlarge and see details.)