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Antibodies

IKKβ antibody (mAb)

Clone: 10A9B6
Catalog No: 40907 Format: 100 µg $340 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1
Purification:Affinity Purified
Applications:IP, WB
Reactivity:Human
Molecular Weight:87 kDa

Applications Table

Western Blot Chromatin IP Immunoprecipitation
1 - 2 µg/ml — 2 µg per IP

for IKKb antibody (mAb) (Clone 10A9B6)Background

IKKβ (IκB Kinase beta subunit) – The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB (NFκB p50 and NFκB p65). The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ).

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IκBα at Ser32 and Ser36. This results ultimately in the degradation of IκB and the activation and nuclear translocation of NFκB.

While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK beta appears to be the principal kinase, as IKKα is not required for activation of the IKK complex and the subsequent degradation of IκBα. Once activated, IKKβ autophosphorylates, which decreases IKK activity, thereby preventing a prolonged activation of inflammation.

for IKKb antibody (mAb) (Clone 10A9B6)Immunogen

This antibody was raised against a 6xHis tag full-length protein corresponding to human IKKβ.

for IKKb antibody (mAb) (Clone 10A9B6)Application Notes

This IKKβ antibody (Clone 10A9B6) has been validated for use in Western blotting (1 - 2 µg/ml dilution) and immunoprecipitation (2 µg per IP). Individual optimization may be required.

for IKKb antibody (mAb) (Clone 10A9B6)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IKKb antibody (mAb) (Clone 10A9B6)Positive Control

Active Motif's Daudi nuclear extract (Catalog No. 36091) can be used as a positive control for IKKβ antibody (Clone 10A9B6).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use IKKβ antibody (mAb).

 
IKKb mAb (Clone 10A9B6) tested by Western blot.

IKKβ mAb (Clone 10A9B6) tested by Western blot.
Immunoprecipitation of IKKβ. 2 µg of IKKβ mAb (Clone 10A9B6) was used to immunoprecipitate IKKβ from 1x106 cells. The immunoprecipitated protein was detected by Western blotting using the same antibody. The IKKβ protein detected was ~87 kDa in size.

for IKKb antibody (mAb) (Clone 10A9B6)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for IKKb antibody (mAb) (Clone 10A9B6)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting