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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1 - 2 µg/ml | — |
for IRAK-2 antibody (pAb)Background
IRAK-2 – Interleukin-1 Receptor-Associated Kinase 2 is one of two serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation to upregulate NFκB (NFκB p50 and NFκB p65) expression.
for IRAK-2 antibody (pAb)Immunogen
This antibody was raised against a peptide corresponding to amino acid residues 546-564 of human IRAK-2.
for IRAK-2 antibody (pAb)Application Notes
This IRAK-2 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for IRAK-2 antibody (pAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for IRAK-2 antibody (pAb)Positive Control
Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for IRAK-2 antibody.
IRAK-2 pAb tested by Western blot.
Detection of IRAK-2 by Western blot analysis. The analysis was performed using 10 µg/lane of HeLa (lane 1) and K-562 (lane 2) nuclear extracts and IRAK-2 pAb at a 2 µg/ml dilution.
for IRAK-2 antibody (pAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for IRAK-2 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
