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Antibodies

MyD88 antibody (pAb)

Aliases: MYD88D
Catalog No: 40914 Format: 100 µg $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:35 kDa
Reactivity:Human

Applications

Western Blot Validated

for MyD88 antibody (pAb)Application Notes


Validated Applications:
WB: 2 - 4 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for MyD88 antibody (pAb)Immunogen

This MyD88 antibody was raised against a synthetic peptide corresponding to amino acid residues 233-248 of human MyD88.

for MyD88 antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for MyD88 antibody (pAb)References

 
MyD88 antibody (pAb) tested by Western blot.

MyD88 pAb tested by Western blot.
MyD88 detection by Western blot. The analysis was performed using Jurkat whole-cell extract and MyD88 pAb at a 2 µg/ml dilution.

for MyD88 antibody (pAb)Background

MyD88 is an adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. MyD88 acts via IRAK1, IRAK2 and TRAF6, leading to NFκB activation, cytokine secretion and the inflammatory response. MyD88 increases IL-8 transcription, and may be involved in myeloid differentiation.

for MyD88 antibody (pAb)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for MyD88 antibody.

for MyD88 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MyD88 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MyD88 antibody (pAb)
 

Data Thumbnails

MyD88 antibody (pAb) tested by Western blot.

Western blot of MyD88 pAb.
(Click image to enlarge and see details.)