RANK antibody (mAb)

for RANK antibody (mAb)Background

RANK (TNFRSF11A, CD265, PDB2, TRANCER) can interact with various TRAF family proteins (TRAF2, TRAF5), through which this receptor induces the activation of NFκB and MAPK8/JNK. RANK and its ligand are important regulators of the interaction between T cells and dendritic cells. RANK is also an essential mediator for osteoclast and lymph node development. Studies of the mouse counterpart suggest that this receptor directly mediates the osteoprotegerin (OPG) ligand (OPGL)-induced oteoclastogenesis in osteoclast precursor cells.

for RANK antibody (mAb)Immunogen

This RANK antibody was raised against a GST-fusion protein containing amino acid residues 326-616 of human RANK.

for RANK antibody (mAb)Application Notes

This RANK antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for RANK antibody (mAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for RANK antibody (mAb)Positive Control

Extracts from 293 cells transfected with RANK cDNA can be used as a positive control for RANK antibody.

for RANK antibody (mAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for RANK antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.