TRAF2 antibody (mAb)

for TRAF2 antibody (mAb)Background

TRAF2 (TNF receptor-associated factor 2, TRAP) directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NFκB (NFκB p65 and NFκB p50). The protein complex formed by TRAF2 and TRAF1 interacts with the inhibitor-of-apoptosis proteins (Livin/ML-IAP for example), and functions as a mediator of the anti-apoptotic signals from TNF receptors. TRAF2 interaction with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation (Caspase-3, Caspase-7, Caspase-8, Caspase-9 and Caspase-14). BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis.

for TRAF2 antibody (mAb)Immunogen

This antibody was raised against a fusion protein corresponding to amino acid residues 215 to 222 of human TRAF2.

for TRAF2 antibody (mAb)Application Notes

This TRAF2 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for TRAF2 antibody (mAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for TRAF2 antibody (mAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for TRAF2 antibody.

for TRAF2 antibody (mAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for TRAF2 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.