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Antibodies

DRAK1 antibody (pAb)

Aliases: STK17A
Catalog No: 40923 Format: 100 µg $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:56 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for DRAK1 antibody (pAb)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for DRAK1 antibody (pAb)Immunogen

This DRAK1 antibody was raised against a peptide corresponding to amino acid residues 5 to 9 of human DRAK1.

for DRAK1 antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for DRAK1 antibody (pAb)References

 
DRAK1 antibody (pAb) tested by Western blot.

DRAK1 pAb tested by Western blot.
Detection of DRAK1 by Western blot. The analysis was performed using MOLT4 (lane 1), A-431 (lane 2) and NIH/3T3 (lane 3) nuclear extracts and DRAK1 pAb at a 2 µg/ml dilution.

for DRAK1 antibody (pAb)Background

Overexpression of DRAK1 (STK17A or DAP kinase-related apoptosis-inducing protein kinase 1) and ZIP kinase can cause the morphological changes typical of apoptosis in NIH/3T3 cells. Both DRAKs and ZIP kinase share significant homology at the amino acid level. The kinase domains of both DRAKs and ZIP kinase are homologous to DAP (Death-associated protein) kinase, which is involved in the apoptotic signaling induced by interferon-γ. Overexpression of DRAKs in NIH/3T3 cells lead to apoptosis. These experiments also indicate that the C-terminal domain of DRAKs are important for induction of apoptosis.

for DRAK1 antibody (pAb)Positive Control

Active Motif's A-431 nuclear extract (Catalog No. 36004) can be used as a positive control for DRAK1 antibody.

for DRAK1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DRAK1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DRAK1 antibody (pAb)
 

Data Thumbnails

DRAK1 antibody (pAb) tested by Western blot.

Western blot of DRAK1 pAb.
(Click image to enlarge and see details.)