DRAK1 antibody (pAb)

for DRAK1 antibody (pAb)Background

Overexpression of DRAK1 (STK17A or DAP kinase-related apoptosis-inducing protein kinase 1) and ZIP kinase can cause the morphological changes typical of apoptosis in NIH/3T3 cells. Both DRAKs and ZIP kinase share significant homology at the amino acid level. The kinase domains of both DRAKs and ZIP kinase are homologous to DAP (Death-associated protein) kinase, which is involved in the apoptotic signaling induced by interferon-γ. Overexpression of DRAKs in NIH/3T3 cells lead to apoptosis. These experiments also indicate that the C-terminal domain of DRAKs are important for induction of apoptosis.

for DRAK1 antibody (pAb)Immunogen

This antibody was raised against a peptide corresponding to amino acid residues 5 to 9 of human DRAK1.

for DRAK1 antibody (pAb)Application Notes

This DRAK1 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for DRAK1 antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for DRAK1 antibody (pAb)Positive Control

Active Motif's A-431 nuclear extract (Catalog No. 36004) can be used as a positive control for DRAK1 antibody.

for DRAK1 antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for DRAK1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.