| Antibody Type: | Monoclonal |
| Isotype: | Mouse IgG1 |
| Purification: | Affinity Purified |
|
| Appl.: | WB |
| Reactivity: | Human |
| Molecular Weight: | 35 kDa |
|
| Western Blot |
Chromatin IP |
| 1 - 2 µg/ml |
Not tested |
Background for Caspase-7 antibody (mAb):
Caspase-7 (
CASP7 or
apoptosis-related cysteine peptidase 7) is involved in the activation cascade of caspases responsible for apoptosis execution. The precursor form of
Caspase-7 is cleaved by
Caspase-3 and Caspase-10, which is activated by cell death stimuli and induces apoptosis.
Caspase-7 cleaves and activates sterol regulatory element binding proteins (SREBPs) and proteolytically cleaves poly(ADP-ribose) polymerase (PARP, see
PARP-1 C-terminal and
PARP-1 N-terminal antibodies).
Immunogen for Caspase-7 antibody (mAb):
This antibody was raised against a recombinant human Caspase-7 protein.
Application Notes for Caspase-7 antibody (mAb):
This Caspase-7 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Buffer:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Caspase-7 mAb tested by Western blot.
Caspase-7 detection by Western blot. The analysis was performed using Jurkat nuclear extracts and Caspase-7 mAb at dilutions of 2 µg/ml (lane 1) and 0.5 µg/ml (lane 2). This antibody only detects the 35 kDa caspase-7 corresponding to pro-caspase-7 in non-apoptotic Jurkat cells.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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