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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1 - 2 µg/ml | — |
for Caspase-8 antibody (mAb)Background
Caspase-8 (CASP8 or apoptosis-related cysteine peptidase 8) is the most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adaptor molecule FADD recruits it to either receptor. Caspase-8 cleaves and activates Caspase-3, Caspase-4, Caspase-6, Caspase-7, Caspase-9 and Caspase-10.
for Caspase-8 antibody (mAb)Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acid residues 217-222 of human Caspase-8.
for Caspase-8 antibody (mAb)Application Notes
This Caspase-8 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution), flow cytometry (0.1 µg) and immunohistochemistry (5 µg/ml dilution). For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for Caspase-8 antibody (mAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Caspase-8 mAb tested by Western blot.
Caspase-8 detection by Western blot. The analysis was performed using Jurkat extracts with Caspase-8 mAb at 2 µg/ml (lane 1) and 0.5 µg/ml (lane 2) dilutions. The antibody only detects 55 kDa caspase-8 in staurosporine-treated (2 hours) Jurkat cells.
for Caspase-8 antibody (mAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Caspase-8 antibody (mAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
