| Antibody Type: | Monoclonal |
| Isotype: | Mouse IgG1 |
| Purification: | Affinity Purified |
|
| Appl.: | FACS, IHC, WB |
| Reactivity: | Human |
| Molecular Weight: | 55 kDa |
|
| Western Blot |
Chromatin IP |
| 1 - 2 µg/ml |
Not tested |
Background for Caspase-8 antibody (mAb):
Caspase-8 (
CASP8 or
apoptosis-related cysteine peptidase 8) is the most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adaptor molecule FADD recruits it to either receptor.
Caspase-8 cleaves and activates
Caspase-3, Caspase-4, Caspase-6,
Caspase-7,
Caspase-9 and Caspase-10.
Immunogen for Caspase-8 antibody (mAb):
This antibody was raised against a synthetic peptide corresponding to amino acid residues 217-222 of human Caspase-8.
Application Notes for Caspase-8 antibody (mAb):
This Caspase-8 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution), flow cytometry (0.1 µg) and immunohistochemistry (5 µg/ml dilution). For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Buffer:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Caspase-8 mAb tested by Western blot.
Caspase-8 detection by Western blot. The analysis was performed using Jurkat extracts with Caspase-8 mAb at 2 µg/ml (lane 1) and 0.5 µg/ml (lane 2) dilutions. The antibody only detects 55 kDa caspase-8 in staurosporine-treated (2 hours) Jurkat cells.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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