Caspase-8 antibody (mAb)

for Caspase-8 antibody (mAb)Background

Caspase-8 (CASP8 or apoptosis-related cysteine peptidase 8) is the most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adaptor molecule FADD recruits it to either receptor. Caspase-8 cleaves and activates Caspase-3, Caspase-4, Caspase-6, Caspase-7, Caspase-9 and Caspase-10.

for Caspase-8 antibody (mAb)Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acid residues 217-222 of human Caspase-8.

for Caspase-8 antibody (mAb)Application Notes

This Caspase-8 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution), flow cytometry (0.1 µg) and immunohistochemistry (5 µg/ml dilution). For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Caspase-8 antibody (mAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for Caspase-8 antibody (mAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Caspase-8 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.