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Antibodies

Caspase-8 antibody (mAb)

Clone: 90A992
Aliases: CASP8
Catalog No: 40930 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:55 kDa
Reactivity:Human

Applications

Western Blot Validated

for Caspase-8 antibody (mAb) (Clone 90A992)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Caspase-8 antibody (mAb) (Clone 90A992)Immunogen

This Caspase-8 antibody was raised against a synthetic peptide corresponding to amino acid residues 217-222 of human Caspase-8.

for Caspase-8 antibody (mAb) (Clone 90A992)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for Caspase-8 antibody (mAb) (Clone 90A992)References

 
Caspase-8 antibody (mAb) tested by Western blot.

Caspase-8 mAb tested by Western blot.
Caspase-8 detection by Western blot. The analysis was performed using Jurkat extracts with Caspase-8 mAb at 2 µg/ml (lane 1) and 0.5 µg/ml (lane 2) dilutions. The antibody only detects 55 kDa caspase-8 in staurosporine-treated (2 hours) Jurkat cells.

for Caspase-8 antibody (mAb) (Clone 90A992)Background

Caspase-8 (CASP8 or apoptosis-related cysteine peptidase 8) is the most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adaptor molecule FADD recruits it to either receptor. Caspase-8 cleaves and activates Caspase-3, Caspase-4, Caspase-6, Caspase-7, Caspase-9 and Caspase-10.

for Caspase-8 antibody (mAb) (Clone 90A992)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Caspase-8 antibody (mAb) (Clone 90A992)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Caspase-8 antibody (mAb)
 

Data Thumbnails

Caspase-8 antibody (mAb) tested by Western blot.

Western blot of Caspase-8 mAb.
(Click image to enlarge and see details.)