| Antibody Type: | Monoclonal |
| Isotype: | Mouse IgG1 |
| Purification: | Affinity Purified |
|
| Appl.: | WB |
| Reactivity: | Human, Mouse |
| Molecular Weight: | 30 kDa |
|
| Western Blot |
Chromatin IP |
| 1 - 2 µg/ml |
Not tested |
Background for Caspase-14 antibody (mAb):
Caspase-14 (
CASP14 or
apoptosis-related cysteine peptidase 14) is thought to be a non-apoptotic caspase involved in epidermal differentiation.
Caspase-14 has been shown to be processed and activated by
Caspase-8 and Caspase 10
in vitro, and also by TNF-Related Apoptosis-Inducing Ligand (
TRAIL)
in vivo.
Immunogen for Caspase-14 antibody (mAb):
This antibody was raised against a synthetic peptide corresponding to amino acid residues 2-18 of mouse Caspase-14.
Application Notes for Caspase-14 antibody (mAb):
This Caspase-14 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Buffer:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Positive Control:
Extracts from mouse spleen can be used as a positive control for Caspase-14 antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Caspase-14 mAb tested by Western blot.
Caspase-14 detection by Western blot. The analysis was performed using 10 µg of mouse spleen cell extract and Caspase-14 mAb at a 2 µg/ml dilution.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
YOUR CART
