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TROY/TAJ antibody (pAb)
| RRID: AB_2793451 |
| Aliases: TNFRSF19, TRADE, TAJ-alpha |
| Catalog No: 40937 | Format: 100 µg | $500 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.5 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
Immunogen
This TROY/TAJ antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 29-44, 204-219, and 409-421 of human TROY/TAJ.
Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
TROY/TAJ pAb tested by Western blot.
TAJ/TROY detection by Western blot. The analysis of TAJ/TROY was performed using TROY/TAJ pAb in T98G, a human glioblastoma cell line extract. A protein band of approximately 46 kDa was detected.
Background
TROY / TAJ – Tumor Necrosis Factor Receptor Superfamily Member 19 is a member of the TNF-receptor superfamily. It is highly expressed during embryonic development and has been shown to interact with TRAF (TRAF2 and TRAF5) family members, and to activate the JNK signaling pathway when overexpressed in cells. This receptor can induce apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development.
Positive Control
Extracts from T98G cells can be used as a positive control for TROY/TAJ antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
