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Antibodies

TRANCE/RANKL antibody (mAb)

Clone: 12A380
Catalog No: 40940 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:40 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for TRANCE/RANKL antibody (mAb) (Clone 12A380)Application Notes


Validated Applications:
WB: 4 - 6 µg/ml dilution

Published Applications: see references

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for TRANCE/RANKL antibody (mAb) (Clone 12A380)Immunogen

This TRANCE/RANKL antibody was raised against a GST-fusion protein containing amino acid residues 1-317 of human TRANCE/RANKL.

for TRANCE/RANKL antibody (mAb) (Clone 12A380)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for TRANCE/RANKL antibody (mAb) (Clone 12A380)References

 
TRANCE/RANKL antibody (mAb) tested by Western blot.

TRANCE/RANKL mAb tested by Western blot.
TRANCE detection by Western blot. The analysis was performed using TRANCE/RANKL mAb and transfected RAW cells.

for TRANCE/RANKL antibody (mAb) (Clone 12A380)Background

TRANCE / RANKLTumor Necrosis Factor-Related Activation-induced Cytokine, also known as Receptor Activator of NFκB Ligand is a member of the tumor necrosis factor (TNF) cytokine family and is a ligand for osteoprotegerin (OPG). TRANCE/RANKL functions as a key factor for osteoclast differentiation and activation, is a key survival factor for dendritic cells and is involved in the regulation of T cell-dependent immune response. Overexpression of this protein leads to an increase of osteoclastogenesis and bone loss. This protein may also have a role in regulating apoptosis, by activating the anti-apoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor 6 (TRAF6).

for TRANCE/RANKL antibody (mAb) (Clone 12A380)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TRANCE/RANKL antibody (mAb) (Clone 12A380)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

TRANCE/RANKL antibody (mAb)
 

Data Thumbnails

TRANCE/RANKL antibody (mAb) tested by Western blot.

Western blot of TRANCE/RANKL mAb.
(Click image to enlarge and see details.)