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Applications Table
| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| 1 - 2 µg/ml | — | Yes |
for D4-GDI antibody (mAb)Background
D4-GDI (Rho GDP dissociation inhibitor beta or ARHGDIB) plays an important role in the activation of the superoxide (O2-)-generating NADPH oxidase of phagocytes. The mechanisms involving cleavage of D4-GDI (Rho GDP) with apoptosis are not presently known. However, activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
for D4-GDI antibody (mAb)Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acid residues in the Fas-induced cleavage site of human D4-GDI.
for D4-GDI antibody (mAb)Application Notes
This D4-GDI antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution), and reported to work in immunoprecipitation. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for D4-GDI antibody (mAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use D4-GDI antibody (mAb).
D4-GDI mAb tested by Western blot.
Detection of D4-GDI by Western blot analysis. HL-60 cells were treated for different time periods with 10 ng/ml of Fas monoclonal antibody. Harvested cells were lysed, resolved by SDS-PAGE and transferred onto a PVDF membrane. The membrane was incubated with the D4-GDI mAb and immunoreactivity was detected by chemiluminescence. The data shows that the D4-GDI antibody specifically detects the 23 kDa cleaved form of D4-GDI in a time-sensitive manner.
for D4-GDI antibody (mAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for D4-GDI antibody (mAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
