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Antibodies

IKKi/IKKε antibody (pAb)

Aliases: IKBKE, IKK-E, IKK-i
Catalog No: 40957 Format: 100 µg $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:80 kDa
Reactivity:Human

Applications

Western Blot Validated

for IKKi/IKKe antibody (pAb)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for IKKi/IKKe antibody (pAb)Immunogen

This IKKi/IKKε antibody was raised against a synthetic peptide corresponding to amino acid residues 175-188, 525-540, and 567-580 of human IKKi/IKKε.

for IKKi/IKKe antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IKKi/IKKe antibody (pAb)References

 
IKKi/IKKε antibody (pAb) tested by Western blot.

IKKi/IKKε pAb tested by Western blot.
IKKi/IKKε detection by Western blot. The analysis was performed using 10 µg of whole-cell extract from Daudi cells and IKKi/IKKε pAb at a 2 µg/ml dilution.

for IKKi/IKKe antibody (pAb)Background

IKKi/IKKεIκB Kinase-epsilon (also called IKKi) is a homolog of the IKK (IκB kinase) catalytic subunits IKKα and IKKβ. IKKε is primarily expressed in immune cells and may be responsible for modulation of NFκB (NFκB p50 and NFκB p65) activity in the immune system.

for IKKi/IKKe antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for IKKi/IKKe antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

IKKi/IKKε antibody (pAb)
 

Data Thumbnails

IKKi/IKKε antibody (pAb) tested by Western blot.

Western blot of IKKi/IKKε pAb.
(Click image to enlarge and see details.)