Livin/ML-IAP antibody (mAb)

for Livin/ML-IAP antibody (mAb)Background

Livin (ML-IAP or BIRC7) protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, Livin (ML-IAP or BIRC7) inhibits Caspase-3 and proteolytic activation of pro-Caspase-9. Isoform 1 of Livin (ML-IAP or BIRC7) blocks staurosporine-induced apoptosis, while isoform 2 blocks etoposide-induced apoptosis.

for Livin/ML-IAP antibody (mAb)Immunogen

This antibody was raised against a GST-fusion protein corresponding to human Livin/ML-IAP.

for Livin/ML-IAP antibody (mAb)Application Notes

This Livin/ML-IAP antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Livin/ML-IAP antibody (mAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for Livin/ML-IAP antibody (mAb)Positive Control

Extracts from cells transfected with human Livin cDNA can be used as a positive control for Livin/ML-IAP antibody.

for Livin/ML-IAP antibody (mAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Livin/ML-IAP antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.