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| Western Blot | Chromatin IP |
|---|---|
| 1 - 2 µg/ml | Not tested. |
for FosB antibody (pAb)Background
FosB (FBJ murine osteosarcoma viral oncogene homolog B or Fos B) is a member of the Fos gene family of leucine zipper proteins that dimerize with the Jun family of proteins to form transcription factor AP-1. AP-1 controls cellular processes such as differentiation, proliferation and apoptosis. Other AP-1 family members consist of c-Fos, Fra-1, Fra-2, c-Jun, JunB, and JunD.
for FosB antibody (pAb)Immunogen
This FosB antibody was raised against a synthetic peptide corresponding to human FosB.
for FosB antibody (pAb)Application Notes
This FosB antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for FosB antibody (pAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for FosB antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for FosB antibody.
FosB pAb tested by Western blot.
Detection of FosB by Western blot. The analysis was performed using HeLa nuclear extract and FosB mAb. A protein of approximate MW of 46 kDa was detected. The lower MW protein detected at 30 kDa may be the isoform of D2D FosB.
for FosB antibody (pAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for FosB antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
