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Antibodies

FosB antibody (mAb)

Clone: 83B1138
Aliases: AP-1, G0S3, GOS3, GOSB
Catalog No: 40960 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:46 kDa
Reactivity:Human

Applications

Western Blot Validated

for FosB antibody (mAb) (Clone 83B1138)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for FosB antibody (mAb) (Clone 83B1138)Immunogen

This FosB antibody was raised against a peptide corresponding to amino acid residues 2-14 of human FosB.

for FosB antibody (mAb) (Clone 83B1138)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for FosB antibody (mAb) (Clone 83B1138)References

 
FosB antibody (mAb) tested by Western blot.

FosB mAb tested by Western blot.
Detection of FosB by Western blot. The analysis was performed using HeLa nuclear extract and FosB mAb. A protein of approximate MW of 46 kDa was detected. The lower MW protein detected at 34 kDa may be the isoform of D2D FosB.

for FosB antibody (mAb) (Clone 83B1138)Background

FosB (FBJ murine osteosarcoma viral oncogene homolog B or Fos B) is a member of the Fos gene family of leucine zipper proteins that dimerize with the Jun family of proteins to form transcription factor AP-1. AP-1 controls cellular processes such as differentiation, proliferation and apoptosis. Other AP-1 family members consist of c-Fos, Fra-1, Fra-2, c-Jun, JunB and JunD.

for FosB antibody (mAb) (Clone 83B1138)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for FosB antibody.

for FosB antibody (mAb) (Clone 83B1138)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for FosB antibody (mAb) (Clone 83B1138)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

FosB antibody (mAb)
 

Data Thumbnails

FosB antibody (mAb) tested by Western blot.

Western blot of FosB mAb.
(Click image to enlarge and see details.)