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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 0.5 - 2 µg/ml | Not recommended. | 1:100 |
for ATF-6 antibody (mAb)Background
ATF-6 (activating transcription factor 6) is a transmembrane transcription factor that acts during endoplasmic reticulum (ER) stress to activate transcription.
for ATF-6 antibody (mAb)Immunogen
This ATF-6 antibody was raised against a partial protein containing amino acid residues 1-273 of human ATF-6.
for ATF-6 antibody (mAb)Application Notes
This ATF-6 antibody has been validated for use in Western blotting (0.5 - 2.0 µg/ml dilution) and immunofluorescence (1:100 dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for ATF-6 antibody (mAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with ATF-6 antibody (mAb).
ATF-6 mAb tested by Western blot.
ATF-6 detection by Western blot. The analysis was performed using ATF-6 mAb and 10 µg 293 nuclear extract. A protein of approximately 90 kDa is detected.
for ATF-6 antibody (mAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for ATF-6 antibody (mAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
