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| Western Blot | Chromatin IP |
|---|---|
| 1 - 2 µg/ml | Not tested. |
for DAP-3 antibody (mAb)Background
DAP-3 (Death Associated Protein 3) potentially binds ATP/GTP and may be a functional partner of the mitoribosomal protein S27. The DAP-3 gene encodes a 28S subunit protein that also participates in apoptotic pathways that are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon.
for DAP-3 antibody (mAb)Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acid residues 51-66 of human DAP-3.
for DAP-3 antibody (mAb)Application Notes
This DAP-3 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for DAP-3 antibody (mAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for DAP-3 antibody (mAb)Positive Control
Active Motif's Jurkat cytoplasmic extract (Catalog No. 36402) can be used as a positive control for DAP-3 antibody.
DAP-3 mAb tested by Western blot.
DAP-3 detection by Western blot. The analysis was performed using 10 µg Jurkat whole-cell extract and the DAP-3 mAb at a 1 µg/ml dilution.
for DAP-3 antibody (mAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for DAP-3 antibody (mAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
