DAP-5 antibody (mAb)

for DAP-5 antibody (mAb)Background

DAP-5 (eIF4G2 or eukaryotic translation initiation factor 4 gamma 2) shares similarity with the C-terminal region of eIF4G, which contains the binding sites for eIF4A and eIF3. eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. DAP-5 appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection.

for DAP-5 antibody (mAb)Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acid residues 796-812 of human DAP-5.

for DAP-5 antibody (mAb)Application Notes

This DAP-5 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for DAP-5 antibody (mAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for DAP-5 antibody (mAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for DAP-5 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.