TRAIL antibody (mAb)

for TRAIL antibody (mAb)Background

TRAIL (TNFSF10, CD253, Apo2L) is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL is an acronym for TNF-Related Apoptosis-Inducing Ligand. Both the transmembrane and soluble recombinant forms of TRAIL can rapidly induce apoptosis in a wide variety of transformed and tumor cell lines, yet no cytotoxicity is observed with normal human cell lines, even though TRAIL and its receptors are expressed at significant levels there, and in most normal tissues in vitro. TRAIL is expressed constitutively in many tissues such as liver, lung, placenta, kidney, spleen, peripheral lymphocyte, etc. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, Caspase-3, and Caspase-8. TRAIL induces cancer apoptosis independently of p53 gene mutations. Two additional TRAIL decoy receptors (DcR1 and DcR2) do not trigger an apoptotic signal in normal cells and have been proposed to confer protection from TRAIL-inducing apoptosis. TRAIL may also play roles in immune responses, autoimmune disorders and HIV infection. TRAIL can interact with five different members of the TNF receptor superfamily, two of which contain a death domain, and can mediate an apoptotic response. TRAIL may also be a marker for progression of cancers.

for TRAIL antibody (mAb)Immunogen

This antibody was raised against a peptide corresponding to amino acid residues 17-35 of human TRAIL.

for TRAIL antibody (mAb)Application Notes

This TRAIL antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution), and reported to work in flow cytometry. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for TRAIL antibody (mAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for TRAIL antibody (mAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for TRAIL antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.