HDAC4 antibody (pAb)

for HDAC4 antibody (pAb)Background

HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).

Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.

for HDAC4 antibody (pAb)Immunogen

This HDAC4 antibody was raised against a synthetic peptide corresponding to amino acid residues 194-209 of human HDAC4.

for HDAC4 antibody (pAb)Application Notes

This HDAC4 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution) and chromatin IP (5 - 10 µg per ChIP). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for HDAC4 antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for HDAC4 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for HDAC4 antibody.

for HDAC4 antibody (pAb)References

This antibody has been used as described below:

for HDAC4 antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HDAC4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.