| Antibody Type: | Monoclonal |
| Isotype: | Mouse IgG1 |
| Purification: | Ascites |
|
| Appl.: | WB |
| Reactivity: | Human |
| Molecular Weight: | 73 kDa |
|
| Western Blot |
Chromatin IP |
| 1:500 |
Not tested |
Background for p73 antibody (mAb):
p73 is a
p53-related tumor protein, with some structural resemblance to
p53, and is therefore considered a tumor-suppressor protein.
p73 has numerous isoforms arising from alternative splicing, resulting in 6 different C-termini. Overexpression of
p73 promotes growth arrest and/or apoptosis in a manner similar to that seen with
p53.
Immunogen for p73 antibody (mAb):
This antibody was raised against a full-length protein corresponding to human p73.
Application Notes for p73 antibody (mAb):
This p73 antibody has been validated for use in Western blotting (1:500 dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. This p73 antibody reacts with an epitope that is located on the N-terminal region of human p73 and does not cross react with p53. The p73 protein may run at 63 kDa in some cell lines due to post-transcriptional modifications.
Buffer:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Positive Control:
Extracts from cells transfected with p73 can be used as a positive control for p73 antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
p73 mAb tested by Western blot.
p73 monoclonal antibody used at a 1:500 dilution against 10 µg of transfected cell lysate.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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