| Antibody Type: | Monoclonal |
| Isotype: | Mouse IgG1 |
| Purification: | Affinity Purified |
|
| Appl.: | WB |
| Reactivity: | Human |
| Molecular Weight: | 63 kDa |
|
| Western Blot |
Chromatin IP |
| 3 - 5 µg/ml |
Not tested |
Background for p63 antibody (mAb):
p63, also known as
TP63 (for
tumor protein p63), is a
p53-related tumor protein with some structural resemblance to
p53, and is therefore considered a tumor-suppressor protein.
p63 has numerous isoforms arising from alternative splicing, resulting in 6 different C-termini. Overexpression of
p63 promotes growth arrest and/or apoptosis in a manner similar to that seen with
p53. It is associated with ectrodactyly-ectodermal dysplasia-cleft syndrome and Hay-Wells syndrome.
Immunogen for p63 antibody (mAb):
This p63 antibody was raised against a synthetic peptide corresponding to human p63.
Application Notes for p63 antibody (mAb):
This p63 antibody has been validated for use in Western blotting (3 - 5 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Buffer:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Positive Control:
Whole-cell extracts from 293 cells can be used as a positive control for p63 antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
YOUR CART
